1/Advantages and disadvantages of using (CARS) and what are the drawbacks of using this technique. is it difficult to apply this technique? 2/The CARS microscope 3/Applications – Biology, Food, Cosmetics ( briefly )+ (add images) 4/focus on (( imaging the living cells with CARS in Three-Dimensional )). I mean the process and procedures of applying this technique in living cells. ( widely) ((this is the most important part in my research)) ( add pictures ) notice/ 1/there are some references in this link , please use them. https://www.dropbox.com/sh/3k85ux0nr171yky/AAACkXPTcgCx7UHV4bGd3E9ma?dl=0 2/please use Harvard style in references and citations.